منابع مشابه
tRNA-isoleucine-tryptophan composite gene.
Transfer-RNA genes in archaea often have introns intervening between exon sequences. The structural motif at the boundary between exon and intron is the bulge-helix-bulge. Computational investigations of these boundary structures in Haloarcula marismortui lead us to propose that tRNA-isoleucine and tRNA-tryptophan genes are co-located. Precise in silico identification of the splice-sites on the...
متن کاملA potato mitochondrial isoleucine tRNA is coded for by a mitochondrial gene possessing a methionine anticodon.
The nucleotide sequence of a potato mitochondrial tRNA(Ile) has been determined. This tRNA has a lysidine-like minor nucleotide in the first position of the anticodon (position 34). The corresponding mitochondrial gene has been cloned and sequenced. This gene has a CAT anticodon which corresponds to methionine. The C residue in the anticodon must therefore be post-transcriptionally modified. Th...
متن کاملIdentity determinants of E. coli tryptophan tRNA.
The first base pair of the acceptor stem A1-U72 and the discriminator base G73, as well as the anticodon nucleotides, characterize the tryptophan tRNA in E. coli. To determine the contribution of these nucleotides to the tryptophan acceptor activity, various transcripts of E. coli tryptophan tRNA mutants were constructed. Substitutions of the discriminator base G73, which is conserved within pr...
متن کاملNonprotein amino acid furanomycin, unlike isoleucine in chemical structure, is charged to isoleucine tRNA by isoleucyl-tRNA synthetase and incorporated into protein.
Nonprotein amino acid furanomycin was found to bind with Escherichia coli isoleucyl-tRNA synthetase (IleRS) almost as tightly as the substrate L-isoleucine. The conformation of furanomycin bound to the enzyme was determined by NMR analyses including the transferred nuclear Overhauser effect method. The conformation of IleRS-bound furanomycin was similar to that of L-isoleucine, although the che...
متن کاملNucleotide sequence of tryptophan tRNA from Bacillus subtilis
B. subtilis tryptophan tRNA was isolated utilizing a dot blot hybridization method (1) and its RNA sequence including a modified nucleotide was determined by 2D TLC (2, 3) and UV spectroscopy (4). As a result, we identified five modified nucleotides, (ribothymidine (T54), pseudouridine (¥55, *31), M-isopentenyladenosine (iA37) and dihydrouridine (D20)). In B.subtUis, it has been reported that a...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 2006
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2005.10.183